This image depicts how fasting remodels the gut epithelium. Shown here are lysosomes in the fasted crypts (Cathepsin D, purple, nuclear DAPI staining in cyan, saturated lysosome staining in white). Fasting upregulates lysosome abundance, especially in the intestinal stem cells located at the the crypt bottom.
This an Apc VillincreER colon tumor. It is a series of 20X images stitched together. Yellow is Lgr5 and Purple is MKI67 (proliferating cells).Mouse colon after endoscopic injection of lentiCre show fluorescent red TDtomato+ cells.
